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    Title: An application of electrospray ionization tandem mass spectrometry to probe the interaction of Ca2+/Mg2+/Zn2+ and Cl- with gramicidin A
    Authors: Sudhir, Putty-reddy;吳慧芬;Wu, Hui-fen;周子聰;Zhou, Zi-cong
    Contributors: 淡江大學物理學系
    Date: 2005-06-15
    Issue Date: 2009-12-31 10:51:37 (UTC+8)
    Publisher: John Wiley & Sons
    Abstract: The peptide, gramicidin A (GrA), has been demonstrated to interact with divalent salts (CaCl2, MgCl2, and ZnCl2) using electrospray ionization mass spectrometry (ESI-MS). The ESI-MS analysis revealed different complexes formed due to the interaction of Val-GrA and Ile-GrA with divalent salts: [Val or Ile-GrA-H+M]+, [Val or Ile-GrA+MCl]+ and [Val or Ile-GrA+M]2+, where M is Ca or Mg or Zn. All these complexes have been subjected to collisionally activated dissociation (CAD). CAD of singly and doubly charged GrA and metal complexes exhibited the losses of water molecules, indicating the ligand preference of GrA. MS/MS and MS3 of [Val or Ile-GrA+MCl]+ resulted in the elimination of chloride ion and water, respectively. The tandem mass spectrometry data of the complex [Val-GrA+MCl]+ suggest that chloride interaction is stronger in the presence of Ca than of Mg and Zn. This study reveals that GrA could interact with Ca, Mg, and Zn in metal ion form as well as in ion pair (MCl) form. The interactions of GrA with Ca support the proposal of a physical basis for the messenger role of Ca (Urry et al., J. Biol. Chem. 1982, 257: 6659–6661).
    Relation: Rapid Communications in Mass Spectrometry 19(11), pp.1517-1521
    DOI: 10.1002/rcm.1947
    Appears in Collections:[物理學系暨研究所] 期刊論文
    [化學學系暨研究所] 期刊論文

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