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    题名: DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium
    作者: Chu, Benjamin;Liu, Tian-bo;Wu, Chun-hung;Liang, De-hai
    贡献者: 淡江大學化學學系
    日期: 2000-12
    上传时间: 2009-12-01 09:01:14 (UTC+8)
    出版者: Totowa, N.J. : Humana Press
    摘要: The development of automated DNA sequencing has drawn the attention of many research groups in recent years, especially for the high-throughput demand of the Human Genome Project (1,2). Automation of DNA sequencing based on standard electrophoresis is the most commonly accepted and used approach. Capillary electrophoresis (CE) using replaceable polymer solution as a rapid separation medium with high-resolution separations can be automated relatively easily when compared with slab-gel electrophoresis. The development of capillary array electrophoresis (CAE) has further enhanced the throughput of DNA sequencing. The application of replaceable polymer solutions over permanent crosslinked gels as a separation medium made CE the best conventional analytical method at this time (3)f because gels tend to be more difficult to handle and have limited column lifetime.
    關聯: Capillary electrophoresis of nucleic acids Methods in Molecular Biology 162, pp.225-238
    DOI: 10.1385/1-59259-055-1:225
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