Apoptosis of human melanoma cells induced by the novel compounds propolin A and propolin B from Taiwenese propolis

doi:10.1016/j.canlet.2006.01.016

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Title:	Apoptosis of human melanoma cells induced by the novel compounds propolin A and propolin B from Taiwenese propolis
Authors:	吳嘉麗;Wu, Chia-li;Chen, Chia-nan;Lin, Jen-kun
Contributors:	淡江大學化學學系
Keywords:	Propolin A;Propolin B;Taiwanese propolis;Apoptosis;Xanthine oxidase;Human melanoma cells
Date:	2007-01-08
Issue Date:	2009-12-01 09:00:33 (UTC+8)
Publisher:	Elsevier Ireland
Abstract:	We recently demonstrated that two new prenylflavanones, propolin A and propolin B, isolated and characterized from Taiwanese propolis, induced cytotoxicity effect in human melanoma A2058 cells and shows a strong capability to scavenge free radicals. In this study, propolin A effectively induced a cytotoxic effect on five different cancer cell lines. Similar results were obtained for propolin B. DNA flow cytometric analysis and DNA fragmentation ladder indicated that propolin A and propolin B actively induced apoptosis in A2058 cells. To address the mechanism of the apoptosis effect of propolin A and propolin B, we evaluated the apoptosis-related proteins in A2058 cells. The levels of procaspase-8, Bid, procaspase-3, DFF45, and PARP were decreased in dose- and time course-dependent manners. Furthermore, also found propolin A and propolin B was capable of releasing cytochrome c from mitochondria to cytosol. The findings suggest that propolin A and propolin B may activate a mitochondria-mediated apoptosis pathway. On the other hand, our data show that propolin B inhibitied xanthine oxidase activity more efficiently than propolin A or CAPE. However, CAPE suppressed ROS-induced DNA strand breakage more efficiently than propolin A or propolin B. All these results indicated that propolin A and propolin B may trigger apoptosis of A2058 cells through mitochondria-dependent pathways and also shown that propolin A and propolin B were strong antioxidants.
Relation:	Cancer Letters 245(1-2), pp.218-231
DOI:	10.1016/j.canlet.2006.01.016
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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