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    題名: Viscosity-adjustable block copolymer for DNA separation by capillary electrophoresis
    作者: 吳俊弘;Wu, Chunhung;Liu, Tianbo;Chu, Benjamin
    貢獻者: 淡江大學化學學系
    日期: 1998-02
    上傳時間: 2009-12-01
    出版者: Wiley-Blackwell
    摘要: The viscosity-adjustable property of F127 block copolymer PEO99PPO69PEO99, PEO and PPO being poly (ethylene oxide) and poly(propylene oxide), respectively, was found to be useful for the development of automated capillary electrophoresis (CE). The polymer solution can form a gel-like structure with sieving ability and can also serve as a dynamic coating material, thereby effectively suppressing the electroosmotic flow induced by the ionization of the silanol group on the quartz capillary inner wall. When applied to CE as a separation medium, F127 block copolymer can provide the advantages of high separation resolution, easy injection and replacement of the triblock copolymer solution and convenient capillary column treatment. High reproducibility of DNA electrophoretic migration time in CE by replenishing F127 solution in acid-washed capillary tubings can be achieved. The relative standard deviation of the DNA migration time is less than 2%. In the investigation of F127 concentration and temperature effects on the performance of DNA separation in CE, we have found that the DNA electrophoretic migration behavior in the F127 gel-like solution cannot be described by any one of the existing models.
    關聯: Electrophoresis 19(2), pp.231-241
    DOI: 10.1002/elps.1150190216
    顯示於類別:[化學學系暨研究所] 期刊論文

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