The determination of acrylamide in nerve tissue homogenates by electron-capture gas chromatography

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Title:	The determination of acrylamide in nerve tissue homogenates by electron-capture gas chromatography
Authors:	薛文發;Sye, Wen-fa;Poole, C. F.;Zlatkis, A. P.;Spencer S.
Contributors:	淡江大學化學學系
Date:	1981-11-06
Issue Date:	2009-12-01
Publisher:	Elsevier
Abstract:	Acrylamide in biological samples can be determined by gas chromatography with electron-capture detection after conversion to its 2,3-dibromopropionamide derivative. The derivatization is carried out in aqueous solution, plasma or tissue homogenates by ionic bromination and the reaction conditions and sample clean-up are described. The detection limit corresponds to 9.5.10(-12)g of acrylamide on column or 8.4.10(-9)g in the final biological extract (0.5 ml). The overall recovery of acrylamide spiked samples at the nanogram level exceeds 80%. It was found that the accumulation of free acrylamide in the sciatic nerve distal region of rats intoxicated with acrylamide was less than 2-8 ppm.
Relation:	Journal of Chromatography A 217, pp.239-245
DOI:	10.1016/S0021-9673(00)88078-4
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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