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    題名: The determination of acrylamide in nerve tissue homogenates by electron-capture gas chromatography
    作者: 薛文發;Sye, Wen-fa;Poole, C. F.;Zlatkis, A. P.;Spencer S.
    貢獻者: 淡江大學化學學系
    日期: 1981-11-06
    上傳時間: 2009-12-01
    出版者: Elsevier
    摘要: Acrylamide in biological samples can be determined by gas chromatography with electron-capture detection after conversion to its 2,3-dibromopropionamide derivative. The derivatization is carried out in aqueous solution, plasma or tissue homogenates by ionic bromination and the reaction conditions and sample clean-up are described. The detection limit corresponds to 9.5.10(-12)g of acrylamide on column or 8.4.10(-9)g in the final biological extract (0.5 ml). The overall recovery of acrylamide spiked samples at the nanogram level exceeds 80%. It was found that the accumulation of free acrylamide in the sciatic nerve distal region of rats intoxicated with acrylamide was less than 2-8 ppm.
    關聯: Journal of Chromatography A 217, pp.239-245
    DOI: 10.1016/S0021-9673(00)88078-4
    顯示於類別:[化學學系暨研究所] 期刊論文

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