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    题名: The conversion of group B red blood cells into group O by an α-d-galactosidase from taro (Colocasia esculenta)
    其它题名: 轉變B型紅血球為O型紅血球
    作者: Chien, Su-fang;Lin-Chu, Marie
    贡献者: 淡江大學化學學系
    日期: 1991-09-18
    上传时间: 2009-12-01
    出版者: Oxford: Pergamon
    摘要: An α-d-galactosidase (EC 3.2.1.21), capable of converting group B into group O red cells, was isolated from the stem portion of taro. It was purified about 3000 fold by gel filtration and ion-exchange chromatography. The blood group-converting activity was demonstrated by hemolysis and hemagglutination studies. This activity is comparable to that of α-d-galactosidase isolated from coffee beans. Taro α-d-galactosidase also hydrolyzes (1→4)- and (1→6)-linked α-d-galactopyranosyl groups from d-galactose-containing glycoconjugates. Taro α-d-galactosidase has a low Km value (0.28mM), a low molecular weight (40 000), and a neutral optimal pH (6.0). At a final enzyme concentration of 30 units/mL in the incubation mixture, the conversion of group B into group O activity was completed within two hours, without apparent changes in the shape of the red cells.
    關聯: Carbohydrate research 217, pp.191-200
    DOI: 10.1016/0008-6215(91)84129-3
    显示于类别:[化學學系暨研究所] 期刊論文

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