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    题名: A novel reusable nanomechanics based protein biosensor with electrical manipulation
    作者: 李世元;Lee, Adam Shih-yuan;Hwang, R.-Z.;Huang, L.-S.;Chang, H.-S.;Wu, C.-W.;Tien, H.-C.;Lin, S.
    贡献者: 淡江大學化學學系
    关键词: Proteins;Biosensors;Immune system;Silicon;Sea surface;Stress;Nanoscale devices;Gold;Nickel;Electrodes
    日期: 2005
    上传时间: 2009-12-01
    出版者: Institute of Electrical and Electronics Engineers (IEEE)
    摘要: A novel reusable biosensor of label-free biomolecular recognition based on the nanomechanics transduction in a micro-fabricated cantilever has been developed. The nanomechanics-based immunoassay biosensor was proven to be repeatedly used with desorption of the pre-bound antigen-antibody by an in-situ alternative electrical field. This new approach of electric manipulation in separating the antigen out of the antibody in a buffer fluid environment was confirmed in nanomechanics-based immunoassay biosensor in which a conventional desorption is long used with high concentrated acid solution in a range of pH 2/spl sim/3. The potential benefit of the electrical means over a conventional pre-bound antigen-antibody desorption of strong acid solution is to maintain long-standing protein activity in multiple uses such that the micro biosensor is reliable and reproducible in every detection. The complete sensing history from a monolayer of biolinker, to antibody and antigen is also first demonstrated in nanomechanics-based immunoassay.
    關聯: Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on, pp.798-801
    DOI: 10.1109/MEMSYS.2005.1454050
    显示于类别:[化學學系暨研究所] 期刊論文

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