淡江大學機構典藏:Item 987654321/25222
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    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/25222


    Title: Specific recognition of DNA bulge structure by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Authors: Chen, Yu-ju;Chen, Po-jui;Wang, Hsueh-ting;Cheng, Chien-chung;鄭建中
    Contributors: 淡江大學化學學系
    Date: 2004-03-30
    Issue Date: 2009-12-01
    Publisher: John Wiley & Sons
    Abstract: This study reports a novel approach utilizing an octahedral CoII(HAPP)(TFA)2 reagent in the presence of H2O2 with analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to serve as an efficient probe for bulged DNA structures. Elucidation of DNA bulge-specific recognition pathways and cleavage mechanisms is demonstrated by characterization of bulge-specific cleavage products and other backbone lesion fragments. The cleavage specificity of CoII(HAPP)(TFA)2/H2O2 arises from sugar oxidative strand scission, for which the position of the abstracted hydrogen is unambiguously determined as the 4'-H of the deoxyribose moiety. Furthermore, differentiation between bulge-specific recognition and diffusion-controlled non-selective cleavage can be clarified through time-dependent MALDI-TOFMS studies. The present results demonstrate that MALDI-TOFMS can be a sensitive and efficient technique for complex mechanistic studies of this kind, providing information for future rational drug design targeting bulged DNA structures.
    Relation: Rapid Communications in Mass Spectrometry 18(6), pp.609-616
    DOI: 10.1002/rcm.1377
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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