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    题名: Specific recognition of DNA bulge structure by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    作者: Chen, Yu-ju;Chen, Po-jui;Wang, Hsueh-ting;Cheng, Chien-chung;鄭建中
    贡献者: 淡江大學化學學系
    日期: 2004-03-30
    上传时间: 2009-12-01
    出版者: John Wiley & Sons
    摘要: This study reports a novel approach utilizing an octahedral CoII(HAPP)(TFA)2 reagent in the presence of H2O2 with analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to serve as an efficient probe for bulged DNA structures. Elucidation of DNA bulge-specific recognition pathways and cleavage mechanisms is demonstrated by characterization of bulge-specific cleavage products and other backbone lesion fragments. The cleavage specificity of CoII(HAPP)(TFA)2/H2O2 arises from sugar oxidative strand scission, for which the position of the abstracted hydrogen is unambiguously determined as the 4'-H of the deoxyribose moiety. Furthermore, differentiation between bulge-specific recognition and diffusion-controlled non-selective cleavage can be clarified through time-dependent MALDI-TOFMS studies. The present results demonstrate that MALDI-TOFMS can be a sensitive and efficient technique for complex mechanistic studies of this kind, providing information for future rational drug design targeting bulged DNA structures.
    關聯: Rapid Communications in Mass Spectrometry 18(6), pp.609-616
    DOI: 10.1002/rcm.1377
    显示于类别:[化學學系暨研究所] 期刊論文

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