A new separation medium for DNA capillary electrophoresis : self-assembly behavior of pluronic polyol E99P69E99 in 1X TBE buffer

doi:10.1016/S0022-3093(98)00570-5

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/25178

請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/25178

題名:	A new separation medium for DNA capillary electrophoresis : self-assembly behavior of pluronic polyol E99P69E99 in 1X TBE buffer
作者:	吳俊弘;Wu, Chunhung;Liu, Tianbo;Chu, Benjamin
貢獻者:	淡江大學化學學系
關鍵詞:	C274;B125;R177
日期:	1998-08-02
上傳時間:	2009-12-01
出版者:	Elsevier
摘要:	The unique sol–gel transition property of E99P69E99 (with E and P being poly(ethylene oxide) and poly(propylene oxide), respectively), a Pluronic polyol F127, could become a useful separation medium in the miniaturization and automation of capillary electrophoresis (CE). Static light scattering (SLS), dynamic light scattering (DLS), and small-angle X-ray scattering were used to study the solution property and the gel-like structures of F127 in a CE buffer (1X TBE) for DNA separation. F127 in the CE forms core-shell micelles with a closed-association process. The critical micelle concentration (cmc) decreases and the micellar association number increases with increasing temperature. At larger solution concentrations, small-angle X-ray scattering results showed that the gel-like structure formed by close packing of the micelles had a face centered cubic (fcc) lattice. In the gel-like region the association number and also the micellar size were not affected by concentration changes. With F127 solution as a separation medium, CE could be performed successfully with a detection length of only 4 cm for separation of double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) fragments.
關聯:	Journal of Non-Crystalline Solids 235-237, pp.605-611
DOI:	10.1016/S0022-3093(98)00570-5
顯示於類別:	[化學學系暨研究所] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
		0Kb	Unknown	322	檢視/開啟
index.html		0Kb	HTML	156	檢視/開啟

在機構典藏中所有的資料項目都受到原著作權保護.

TAIR相關文章

資料載入中.....