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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/25178


    Title: A new separation medium for DNA capillary electrophoresis : self-assembly behavior of pluronic polyol E99P69E99 in 1X TBE buffer
    Authors: 吳俊弘;Wu, Chunhung;Liu, Tianbo;Chu, Benjamin
    Contributors: 淡江大學化學學系
    Keywords: C274;B125;R177
    Date: 1998-08-02
    Issue Date: 2009-12-01
    Publisher: Elsevier
    Abstract: The unique sol–gel transition property of E99P69E99 (with E and P being poly(ethylene oxide) and poly(propylene oxide), respectively), a Pluronic polyol F127, could become a useful separation medium in the miniaturization and automation of capillary electrophoresis (CE). Static light scattering (SLS), dynamic light scattering (DLS), and small-angle X-ray scattering were used to study the solution property and the gel-like structures of F127 in a CE buffer (1X TBE) for DNA separation. F127 in the CE forms core-shell micelles with a closed-association process. The critical micelle concentration (cmc) decreases and the micellar association number increases with increasing temperature. At larger solution concentrations, small-angle X-ray scattering results showed that the gel-like structure formed by close packing of the micelles had a face centered cubic (fcc) lattice. In the gel-like region the association number and also the micellar size were not affected by concentration changes. With F127 solution as a separation medium, CE could be performed successfully with a detection length of only 4 cm for separation of double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) fragments.
    Relation: Journal of Non-Crystalline Solids 235-237, pp.605-611
    DOI: 10.1016/S0022-3093(98)00570-5
    Appears in Collections:[化學學系暨研究所] 期刊論文

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