English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 58323/91877 (63%)
造訪人次 : 14380653      線上人數 : 135
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/25005

    題名: Homopolyvalent antibody–antigen interaction kinetic studies with use of a dual-polarization interferometric biosensor
    作者: 李世元;Lee, Adam Shih-yuan;Lin, Shiming;Lin, Yin-hang;Sheu, Bor-ching;Hsu, Su-ming;Lee, Chih-kung
    貢獻者: 淡江大學化學學系
    關鍵詞: Dual-polarization interferometry;C-reactive protein;Binding constant;Affinity;Stoichiometry;Homopolyvalence
    日期: 2006-12-01
    上傳時間: 2009-12-01
    出版者: Elsevier
    摘要: We used dual-polarization interferometry (DPI) to study the interaction kinetics between a ‘homopolyvalent’ antigen (Ag) and a monoclonal antibody (Ab). A model system, which uses a monoclonal Ab against a homopentameric Ag, C-reactive protein (CRP), is presented with principle and experiments for the study of the interactions between an Ab and an Ag that has multiple identical epitopes. This allows evaluation of the dissociation constant (KD) and of the binding stoichiometry by DPI based on measurements of phase changes of Ab–Ag complexes in the transverse magnetic (TM) and transverse electric (TE) polarization modes. The average experimental value of KD found by the DPI technique for anti-CRP Ab was shown to be in close agreement with the value obtained by an indirect competition-enzyme-linked immunosorbent assay (ELISA). Moreover, the total number of Ab combining sites on the DPI sensor chip was calculated, and the binding stoichiometry of the surface Ag–Ab complex was obtained. This study illustrates the advantages of the DPI method in biosensing in its capacity for simultaneous evaluation of the thickness and refractive index (density, mass) of adsorbed layers. This allowed a comprehensive analysis of affinity reactions between an Ab having two binding sites and a multi-sited Ag.
    關聯: Biosensors and Bioelectronics 22(5), pp.715-721
    DOI: 10.1016/j.bios.2006.02.011
    顯示於類別:[化學學系暨研究所] 期刊論文


    檔案 大小格式瀏覽次數



    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋