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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/25001

    Title: A Fluorescent Carbazole Derivative: High Sensitivity for Quadruplex DNA
    Authors: Chang, C. C.;Wu, J. Y.;Chien, C. W.;Wu, W. S.;Liu, H.;Kang, C. C.;余良杰;Yu, Liang-jye;Chang, D. C.
    Contributors: 淡江大學化學學系
    Date: 2003-10-04
    Issue Date: 2009-12-01
    Publisher: American Chemical Society (ACS)
    Abstract: We have synthesized a novel molecule, 3,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide (BMVC), for recognizing specific quadruplex structures, particularly the quadruplex of human telomeric sequence d(T2AG3)4. The fluorescence intensity of the BMVC molecule increases from 1 to almost 2 orders of magnitude upon interacting with various DNAs. At a concentration of BMVC of 10 μM, fluorescence bands with different colors of BMVC in electrophoresis gels of various DNAs can be observed. The fluorescence of BMVC can be used to discriminate between duplex and quadruplex DNAs. At the low concentration of 0.1 μM BMVC in prestained gels, the fluorescence is observed in the presence of quadruplexes with anti−anti−anti−anti and anti−anti−syn−syn arrangements. However, no fluorescence band is detected upon interacting with duplexes and quadruplexes with anti−syn−anti−syn arrangement. Moreover, the sensitivity assays show that as little as 0.2 pmol of quadruplex of d(T2AG3)4 can be revealed by BMVC.
    Relation: Analytical Chemistry 75(22), pp.6177-6183
    DOI: 10.1021/ac034789i
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

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