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    題名: A Fluorescent Carbazole Derivative: High Sensitivity for Quadruplex DNA
    作者: Chang, C. C.;Wu, J. Y.;Chien, C. W.;Wu, W. S.;Liu, H.;Kang, C. C.;余良杰;Yu, Liang-jye;Chang, D. C.
    貢獻者: 淡江大學化學學系
    日期: 2003-10-04
    上傳時間: 2009-12-01
    出版者: American Chemical Society (ACS)
    摘要: We have synthesized a novel molecule, 3,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide (BMVC), for recognizing specific quadruplex structures, particularly the quadruplex of human telomeric sequence d(T2AG3)4. The fluorescence intensity of the BMVC molecule increases from 1 to almost 2 orders of magnitude upon interacting with various DNAs. At a concentration of BMVC of 10 μM, fluorescence bands with different colors of BMVC in electrophoresis gels of various DNAs can be observed. The fluorescence of BMVC can be used to discriminate between duplex and quadruplex DNAs. At the low concentration of 0.1 μM BMVC in prestained gels, the fluorescence is observed in the presence of quadruplexes with anti−anti−anti−anti and anti−anti−syn−syn arrangements. However, no fluorescence band is detected upon interacting with duplexes and quadruplexes with anti−syn−anti−syn arrangement. Moreover, the sensitivity assays show that as little as 0.2 pmol of quadruplex of d(T2AG3)4 can be revealed by BMVC.
    關聯: Analytical Chemistry 75(22), pp.6177-6183
    DOI: 10.1021/ac034789i
    顯示於類別:[化學學系暨研究所] 期刊論文

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