淡江大學機構典藏:Item 987654321/24938
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 64187/96966 (66%)
Visitors : 11335851      Online Users : 104
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/24938


    Title: DNA Hydrolysis Catalyzed by Tris(diisopropyl-1,4,7-triazacyclononanes)Ethane Metal Complexes
    Authors: 鄭建中;Cheng, Chien-chung;Cheng, Chien-chung;Huang, Yan-chen;Liu, Ming-chan
    Contributors: 淡江大學化學學系
    Keywords: DNA hydrolysis;Triazacyclononane;Cerium;Copper;Trinuclear metal complex.
    Date: 2004-11-01
    Issue Date: 2013-07-29 13:48:33 (UTC+8)
    Publisher: 臺北市：中國化學學會
    Abstract: Novel trinuclear coordinated ligands were designed using three of 1,4,7-triazacyclononane moieties with a tribromo linker. These ligands show greater capability and potency in association with various metal ions. Tris(diisopropyl-1,4,7-triazacyclononanes)ethane (2) associated with Cu2+ and Ce4+ ions shows better reactivity to achieve DNA hydrolysis at 37 °C at pH 8 for 5 hr. Using high resolution of polyacrylamide gel electrophoresis, DNA cleavage reaction in Tris-HCl buffer causes a nicking site at the single-stranded region. Alternatively, the reaction in HEPES buffer shows a random DNA cleavage included the double-stranded region. Hydrolysis of phosphodiester bond mediated by trinuclear metal complex is capable of performing at the physiological temperature 37 °C, but it has a lower reactivity compared to mono- and di-nuclear metal complexes. The result suggests that trinuclear metal complexes have a bulky environment to prevent from going toward the phosphodiester bond. The design of an efficient chemical hydrolyase will be a challenge in order to construct a coordinate ligand with the enhancement of hydrolytic activity and the reduction of the steric environment.
    Relation: Journal of the Chinese Chemical Society=中國化學會會誌 51(5B), pp.1201-1208
    DOI: 10.1002/jccs.200400176
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

    Files in This Item:

    File SizeFormat
    index.html0KbHTML119View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback