淡江大學機構典藏:Item 987654321/24931
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 60882/93651 (65%)
造访人次 : 1191154      在线人数 : 21
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/24931


    题名: Differentiate RNA single-stranded region of the branched structures and hairpin loops by an octahedral cobalt(II) complex
    作者: 鄭建中;Cheng, Chien-chung, Hung, Kuo-chan;Huang-Fu, Wei-chun
    贡献者: 淡江大學化學學系
    关键词: Internal loop;Hairpin loop;Bulge;RNA cleavage;Cobalt
    日期: 2005-12-01
    上传时间: 2009-12-01
    出版者: 中國化學會
    摘要: Single-stranded RNA molecules usually include secondary structural elements such as bulges, internal loops, and hairpin loops. These RNA secondary structural elements are often essential for the biological activity and functions of the RNA molecule. Chemical probe CoII(HAPP)(TFA)2 in the presence of H2O2 is found to differentiate single-stranded RNA from branched structures and hairpin loops. This study uses CoII(HAPP)(TFA)2 to analyze the structures of two RNA molecules: a fragment of HIV TAR RNA (TAR-27) and the catalytic domain 5 of group II intron (D5-29). The electrophoretic mobility of TAR-27 does not shift in the presence of CoII(HAPP)(TFA)2, suggesting that the reagent does not change the conformation of RNA substrate. Cleavage of the RNA substrates by CoII(HAPP)(TFA)2 unambiguously differentiated RNA internal looped structures from hairpin loops. The results show that CoII(HAPP)(TFA)2 is a sensitive, informative and convenient tool for analysis of RNA secondary structures.
    關聯: Journal of the Chinese Chemical Society 52(6), pp.1291-1297
    DOI: 10.1002/jccs.200500186
    显示于类别:[化學學系暨研究所] 期刊論文

    文件中的档案:

    档案 大小格式浏览次数
    0KbUnknown209检视/开启

    在機構典藏中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回馈