Differentiate RNA single-stranded region of the branched structures and hairpin loops by an octahedral cobalt(II) complex

doi:10.1002/jccs.200500186

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Title:	Differentiate RNA single-stranded region of the branched structures and hairpin loops by an octahedral cobalt(II) complex
Authors:	鄭建中;Cheng, Chien-chung, Hung, Kuo-chan;Huang-Fu, Wei-chun
Contributors:	淡江大學化學學系
Keywords:	Internal loop;Hairpin loop;Bulge;RNA cleavage;Cobalt
Date:	2005-12-01
Issue Date:	2009-12-01
Publisher:	中國化學會
Abstract:	Single-stranded RNA molecules usually include secondary structural elements such as bulges, internal loops, and hairpin loops. These RNA secondary structural elements are often essential for the biological activity and functions of the RNA molecule. Chemical probe CoII(HAPP)(TFA)2 in the presence of H2O2 is found to differentiate single-stranded RNA from branched structures and hairpin loops. This study uses CoII(HAPP)(TFA)2 to analyze the structures of two RNA molecules: a fragment of HIV TAR RNA (TAR-27) and the catalytic domain 5 of group II intron (D5-29). The electrophoretic mobility of TAR-27 does not shift in the presence of CoII(HAPP)(TFA)2, suggesting that the reagent does not change the conformation of RNA substrate. Cleavage of the RNA substrates by CoII(HAPP)(TFA)2 unambiguously differentiated RNA internal looped structures from hairpin loops. The results show that CoII(HAPP)(TFA)2 is a sensitive, informative and convenient tool for analysis of RNA secondary structures.
Relation:	Journal of the Chinese Chemical Society 52(6), pp.1291-1297
DOI:	10.1002/jccs.200500186
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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