数据加载中.....
|
jsp.display-item.identifier=請使用永久網址來引用或連結此文件:
https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/24883
|
| 题名: | Comparison of radical scavenging activity, cytotoxic effects and apoptosis induction in human melanoma cells by Taiwanese propolis from different sources |
| 作者: | 吳嘉麗;Wu, Chia-li;Chen, Chia-nan;Weng, Meng-shih;Lin, Jen-kun |
| 贡献者: | 淡江大學化學學系 |
| 日期: | 2004-09 |
| 上传时间: | 2009-12-01 |
| 出版者: | New York: Oxford University Press |
| 摘要: | Propolis is a sticky substance that is collected from plants by honeybees. We previously demonstrated that propolins A, B, C, D, E and F, isolated from Taiwanese propolis (TP), could effectively induce human melanoma cell apoptosis and were strong antioxidant agents. In this study, we evaluated TP for free radical scavenging activity by DPPH (1,2-diphenyl-2-picrylhydrazyl). The phenolic concentrations were quantified by the Folin–Ciocalteu method. The apoptosis trigger activity in human melanoma cells was evaluated. TP contained a higher level of phenolic compounds and showed strong capability to scavenge free radicals. Additionally, TP1g, TP3, TP4 and TP7 exhibited a cytotoxic effect on human melanoma cells, with an IC50 of ∼2.3, 2.0, 3.3 and 3.3 μg/ml, respectively. Flow cytometric analysis for DNA fragmentation indicated that TP1g, TP2, TP3 and TP7 could induce apoptosis in human melanoma cells and there is a marked loss of cells from the G2/M phase of the cell cycle. To address the mechanism of the apoptosis effect of TP, we evaluated its effects on induction of apoptosis-related proteins in human melanoma cells. The levels of procaspase-3 and PARP [poly(ADP-ribose) polymerase] were markedly decreased. Furthermore, propolins A, B, C, D, E and F in TP were determined using HPLC. The results indicate that TP is a rich source of these compounds. The findings suggest that TP induces apoptosis in human melanoma cells due to its high level of propolins. |
| 關聯: | Evidenced-based Complementary and Alternative Medicine 1(2), pp.175-185 |
| DOI: | 10.1093/ecam/neh034 |
| 显示于类别: | [化學學系暨研究所] 期刊論文
|
文件中的档案:
| 档案 |
描述 |
大小 | 格式 | 浏览次数 |
|---|
| 1741-427X_1(2)p175-185.pdf | | 246Kb | Adobe PDF | 268 | 检视/开启 | | index.html | | 0Kb | HTML | 285 | 检视/开启 |
|
在機構典藏中所有的数据项都受到原著作权保护.
|
