淡江大學機構典藏:Item 987654321/24863
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 64178/96951 (66%)
Visitors : 9304554      Online Users : 210
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/24863


    Title: Characterization of the PEO−PPO−PEO Triblock Copolymer and Its Application as a Separation Medium in Capillary Electrophoresis
    Authors: 吳俊弘;Wu, Chunhung;Liu, T.;Chu, B.;Schenider, D. K.;Graziano, V.
    Contributors: 淡江大學化學學系
    Date: 1997-08-11
    Issue Date: 2009-12-01
    Publisher: ACS
    Abstract: The Pluronic polyol F127, PEO99PPO69PEO99 (PEO and PPO being poly(ethylene oxide) and poly(propylene oxide), respectively) has the potential to be used as an effective separation medium in capillary electrophoresis (CE) for the separation of biomacromolecules such as DNA fragments and proteins. Static light scattering (SLS), dynamic light scattering (DLS), small-angle neutron scattering (SANS), and small-angle X-ray scattering (SAXS) were used to characterize the solution properties and the microstructures of F127 in the same electrophoresis buffer used in CE. In the dilute solution region, F127 in CE buffer forms micelles similar to that in water. At high solution concentrations, micelles tend to pack into some crystalline forms with relatively well-structured PPO centers. By using a combination of SANS and SAXS results, we are able to conclusively determine the gellike structure to have a face-centered cubic lattice. The effects of solvent, polymer concentration, temperature, and sample preparation procedure on the gel structure were studied. In the gellike region the aggregation number and also the micellar size are not sensitive to both the concentration change and the temperature change. The results of DNA electrophoretic migration in F127 gels also support these findings.
    Relation: Macromolecules 30(16), pp.4574-4583
    DOI: 10.1021/ma9701088
    Appears in Collections:[Graduate Institute & Department of Chemistry] Journal Article

    Files in This Item:

    File Description SizeFormat
    0KbUnknown307View/Open
    Characterization of the PEO−PPO−PEO Triblock Copolymer and Its Application as a Separation Medium in Capillary Electrophoresis.pdf421KbAdobe PDF0View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback