English  |  正體中文  |  简体中文  |  Items with full text/Total items : 51258/86283 (59%)
Visitors : 8014500      Online Users : 129
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/19585


    Title: Movement disorder and neuromuscular change in zebrafish embryos after exposure to caffeine
    Authors: 陳曜鴻;Chen, Yau-hung;Huang, Yi-hui;溫啟仲;Wen, Chi-chung;Wang, Yun-hsin;Chen, Wei-li;Chen, Li-chao;Tsay, Huey-jen
    Contributors: 淡江大學化學學系
    Keywords: Acetylcholine receptor;Caffeine;Embryonic development;Motor neuron;Muscle;Zebrafish
    Date: 2008-09
    Issue Date: 2010-08-10 09:56:42 (UTC+8)
    Publisher: Philadelphia: Elsevier Inc.
    Abstract: Though caffeine is broadly distributed in many plants and foods, little is known about the teratogenic effects of caffeine during early embryonic development. Here, we used zebrafish as a model to test toxicity and teratogenicity since they have transparent eggs, making the organogenesis of zebrafish embryos easier to observe. When the exposure doses of caffeine were less than 150 ppm (17.5, 35, 50, 100 and 150 ppm), the zebrafish embryos exhibited no significant differences in survival rates after comparison with vehicle-control (0 ppm) group. As the exposure dosages increased, the survival rates decreased. No embryos survived after treatment with 300 ppm caffeine or higher dosages. The most evident change in embryos treated with caffeine was a shorter body length (vehicle-control: 3.26 ± 0.01 mm, n = 49; vs 150 ppm of caffeine: 2.67 ± 0.03 mm, n = 50). In addition, caffeine-treated embryos exhibited significantly reduced tactile sensitivity frequencies of touch-induced movement (vehicle-control: 9.93 ± 0.77 vs 17.5–150 ppm caffeine: 5.37 ± 0.52–0.10 ± 0.06). Subtle changes are easily observed by staining with specific monoclonal antibodies F59, Znp1 and Zn5 to detect morphological changes in muscle fibers, primary motor axons and secondary motor axon projections, respectively. Our data show that the treatment of caffeine leads to misalignment of muscle fibers and motor neuron defects, especially secondary motor neuron axonal growth defects.
    Relation: Neurotoxicology and Teratology 30(5), pp.440-447
    DOI: 10.1016/j.ntt.2008.04.003
    Appears in Collections:[化學學系暨研究所] 期刊論文
    [數學學系暨研究所] 期刊論文

    Files in This Item:

    File Description SizeFormat
    0892-0362_30(5)p440-447.pdf4460KbAdobe PDF294View/Open
    index.html0KbHTML78View/Open
    index.html0KbHTML59View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback