English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 62796/95837 (66%)
造访人次 : 3641518      在线人数 : 302
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/19580


    题名: Spatiotemporal expression of zebrafish D-amino acid oxidase during early embryogenesis
    作者: Chen, Yau-hung;Chen, Wei-li;Wang, Yun-hsin;Huang, Mei-yun;Chern, Ming-kai
    贡献者: 淡江大學生命科學研究所
    关键词: D-amino acid oxidase;D-serine;Fish;Flavoenzyme;Maternal effect;Pronephric duct
    日期: 2007-04
    上传时间: 2009-11-05 16:37:49 (UTC+8)
    出版者: Dordrecht: Springer Netherlands
    摘要: D-amino acid oxidase (DAO, EC 1.4.3.3) is one of the flavoenzymes in peroxisomes catalyzing the oxidation of D-amino acids to α-imino acids. By reverse transcription-polymerase chain reaction, DAO cDNA was cloned from the mRNA of zebrafish embryos. The deduced zebrafish DAO amino acid sequence revealed a 348-amino acid polypeptide containing two FAD-binding sites (amino acids positions 7G to 12G, and 236V to 247W), and a peroxisomal targeting signal sequence (346Ser-347Arg-348Leu) at the C-terminal end. Following comparison of the sequences, we found that the zebrafish DAO polypeptide shared the sequence identities of 93%, 79%, 77%, 77%, 46%, 43% and 25% of the reported DAO of carp, human, mouse, pig, Streptomyces coelicor, Rhodotorula gracilis and Schizosaccharomyces pombe, respectively. Whole-mount in situ hybridization revealed that dao was a maternally inherited gene and that its expression was first observed in the zygote period, gradually down-regulated from the cleavage period to the early blastula stages, and had declined to an undetectable level at 6 h post-fertilization (hpf). At 18-hpf, the zebrafish dao transcripts were detected in a very weak manner, and their expression was restricted in the future head and brain regions as well as in the yolk syncytial layers of the yolk and yolk extension regions. At later stages, dao expression was detected in the brain, gut, swimming bladder, pronephric duct, optic tectum, ventrolateral tract and in the floor plate of the developing spinal cord. The enzymatic activities of DAO were also examined and showed that the DAO enzyme activities were expressed in a dynamic and stage-dependent manner: first it was detected at the 1-cell stage at an extremely low level (0.007 ± 0.005 U/mg), reached the maximum amount at 6 days post-fertilization (dpf) (0.323 ± 0.04 U/mg), and gradually down-regulated to the basal level (0.019 ± 0.005 U/mg) in a 1-month juvenile. From these observations, we conclude that zebrafish dao is a maternally inherited gene, and that its expression was restricted in some neurons, gut, and pronephros and could be used as a good marker for the study of pronephridial development.
    關聯: Fish Physiology and Biochemistry 33(1), pp.73-80
    DOI: 10.1007/s10695-006-9118-1
    显示于类别:[生命科學研究所] 期刊論文

    文件中的档案:

    档案 大小格式浏览次数
    index.html0KbHTML61检视/开启

    在機構典藏中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回馈