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    题名: Inactivation of zebrafish mrf4 leads to myofibril misalignment and motor axon growth disorganization
    作者: 陳曜鴻;Chen, Yau-hung;Wang, Y. H.;Li, C. K.;Lee, G. H.;Tsay, H. J.;Tsai, H. J.
    贡献者: 淡江大學生命科學研究所
    关键词: alternative splicing;mrf4;muscle regulatory factor;myf5;zebrafish
    日期: 2008-04
    上传时间: 2009-11-05 16:37:32 (UTC+8)
    出版者: Hoboken: John Wiley & Sons, Inc.
    摘要: Mrf4 is a basic helix-loop-helix (bHLH) transcription factor associated with myogenesis. Two mrf4 transcripts, mrf4_tv1 and mrf4_tv2, were identified in zebrafish generated by alternative splicing. To study their biological functions, we separately injected the Mrf4-morpholinos, including MO1 (mrf4_tv1:mrf4_tv2 knockdown), MO2+MO3 (mrf4_tv1:mrf4_tv2 knockdown), MO3 (mrf4_tv1 knockdown), and MO4 (mrf4 _tv2 knockdown), into zebrafish embryos to observe mrf4 gene knockdown phenotypes. No phenotypic abnormalities were observed following injection with 0.5 ng of MOI but those injected with 4.5, 9, or 13.5 ng displayed curved-body phenotypes, such as indistinct somite boundaries, and a lack of uniformly sized cell blocks. Similar results were also observed in the (MO2+MO3)-, MO3-, and MO4-injected groups. To further investigate the molecular mechanisms that lead to curved-body phenotypes, we stained embryos with alpha-bungrotoxin and specific monoclonal antibodies F59, Znp1, and Zn5 to detect morphological changes in acetyl-choline receptor (AChR) clusters, muscle fibers, common path of the primary neurons, and secondary neurons axonal projections, respectively. Our results show that the muscle fibers of mrf4-(tvl:tv2)morphant aligned disorderly and lost their integrity and attachment, while the defects became milder in either mrf4_tvl-morphant or mrf4_tv2-morphant. On the other hand, reduced axonal projections and AChR clusters were found in both mrf4_tv2-morphant and mrf4_(tv1:tv2)-morphant but distributed normally in the mrf4_tvl-morphant. We conclude that Mrf4_tv2 is involved in alignment of muscle fibers, and Mrf4_tvl might have cooperative function with Mrf4_tv2 in muscle fiber alignment, without affecting the musclenerve connection.
    關聯: Developmental Dynamics 237(4), pp.1043-1050
    DOI: 10.1002/dvdy.21478
    显示于类别:[生命科學研究所] 期刊論文

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