ShyA, a membrane protein for proper septation of hyphae in Streptomyces

doi:10.1016/j.bbrc.2006.02.178

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Title:	ShyA, a membrane protein for proper septation of hyphae in Streptomyces
Authors:	Tseng, Shun-fu;Huang, Tzu-wen;Chen, Carton W.;Chern, Ming-kai;Tam, Ming F.;Teng, Shu-chun
Contributors:	淡江大學生命科學研究所
Keywords:	Streptomyces;Actinomycetes;Hyphae;Spore;Membrane protein
Date:	2006-05-05
Issue Date:	2009-11-05 16:37:12 (UTC+8)
Publisher:	Maryland Heights: Elsevier Inc
Abstract:	The life cycle of Streptomyces involves the formation of filamentous substrate and aerial hyphae. Following cessation of growth of an aerial hypha, multiple septation occurs at the tip to produce a chain of unigenomic spores. A gene, shyA, which influences several aspects of this growth, was isolated and partially characterized in Streptomyces coelicolor. The gene product is a representative of a well-conserved family of small actinomycete proteins. The shyA mutant sporulates normally but displays hyper septum formation and altered spore-chain morphology. Biochemical separation experiments and immunofluorescence staining demonstrated that the shyA gene product locates at cell membranes. Moreover, yeast two-hybrid screen and GST-pull-down assay showed that ShyA can interact with itself. Altogether, ShyA belongs to a new family of membrane-associated proteins which plays a role in morphological differentiation in actinomycetes.
Relation:	Biochemical and Biophysical Research Communications 343(2), pp.369-377
DOI:	10.1016/j.bbrc.2006.02.178
Appears in Collections:	[Graduate Institue of Life Sciences] Journal Article

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