Conversion of squid pen by using Serratia sp. TKU020 fermentation for the production of enzymes, antioxidants, and N-acetyl chitooligosaccharides

doi:10.1016/j.procbio.2009.04.006

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/18873

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题名:	Conversion of squid pen by using Serratia sp. TKU020 fermentation for the production of enzymes, antioxidants, and N-acetyl chitooligosaccharides
作者:	Wang, San-Lang;Liou, Jen-Yi;Liang, Tzu-Wen;Liu, Kao-Cheng
贡献者:	淡江大學化學學系
关键词:	Chitinase;Chitosanase;Protease;Antioxidant activity;N-Acetyl chitooligosaccharides;Serratia sp.
日期:	2009-08
上传时间:	2013-02-27 09:31:54 (UTC+8)
出版者:	Camden: Elsevier Ltd
摘要:	A chitinase (CHT), a chitosanase (CHS) and a protease (PRO) were purified from the culture supernatant of Serratia sp. TKU020 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHT, CHS and PRO determined by SDS-PAGE were approximately 65 kDa, 55 kDa and 55 kDa, respectively. CHT and CHS were inhibited by Mn2+, EDTA and PRO was inhibited by Mg2+, EDTA. The antioxidant activity of TKU020 culture supernatant was 78% (DPPH scavenging ability). N-Acetylglucosamine (GlcNAc) and N-acetyl chitobiose (GlcNAc)2 were also produced from the culture supernatant by using TKU020 strain fermentation. The maximum production of GlcNAc and (GlcNAc)2 was 1.3 mg/mL and 2.7 mg/mL, respectively, after 4 days of fermentation. With this method, we have shown that squid pen wastes can be utilized and it is effective in the production of enzymes, antioxidants, and N-acetyl chitooligosaccharides, facilitating its potential use in industrial applications and functional foods.
關聯:	Process Biochemistry 44(8), pp.854-861
DOI:	10.1016/j.procbio.2009.04.006
显示于类别:	[化學學系暨研究所] 期刊論文

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