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    Title: Conversion of squid pen by using Serratia sp. TKU020 fermentation for the production of enzymes, antioxidants, and N-acetyl chitooligosaccharides
    Authors: Wang, San-Lang;Liou, Jen-Yi;Liang, Tzu-Wen;Liu, Kao-Cheng
    Contributors: 淡江大學化學學系
    Keywords: Chitinase;Chitosanase;Protease;Antioxidant activity;N-Acetyl chitooligosaccharides;Serratia sp.
    Date: 2009-08
    Issue Date: 2013-02-27 09:31:54 (UTC+8)
    Publisher: Camden: Elsevier Ltd
    Abstract: A chitinase (CHT), a chitosanase (CHS) and a protease (PRO) were purified from the culture supernatant of Serratia sp. TKU020 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHT, CHS and PRO determined by SDS-PAGE were approximately 65 kDa, 55 kDa and 55 kDa, respectively. CHT and CHS were inhibited by Mn2+, EDTA and PRO was inhibited by Mg2+, EDTA. The antioxidant activity of TKU020 culture supernatant was 78% (DPPH scavenging ability). N-Acetylglucosamine (GlcNAc) and N-acetyl chitobiose (GlcNAc)2 were also produced from the culture supernatant by using TKU020 strain fermentation. The maximum production of GlcNAc and (GlcNAc)2 was 1.3 mg/mL and 2.7 mg/mL, respectively, after 4 days of fermentation. With this method, we have shown that squid pen wastes can be utilized and it is effective in the production of enzymes, antioxidants, and N-acetyl chitooligosaccharides, facilitating its potential use in industrial applications and functional foods.
    Relation: Process Biochemistry 44(8), pp.854-861
    DOI: 10.1016/j.procbio.2009.04.006
    Appears in Collections:[化學學系暨研究所] 期刊論文

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