Purification and characterization of a chitosanase from a nattokinase producing strain Bacillus subtilis TKU007

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/18871

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題名:	Purification and characterization of a chitosanase from a nattokinase producing strain Bacillus subtilis TKU007
作者:	Wang, San-Lang;Yeh, Pe-Yi
貢獻者:	淡江大學化學學系
關鍵詞:	Bacillus subtilis;Chitosanase;Nattokinase;Surfactant stable;Shrimp shell wastes
日期:	2008-02
上傳時間:	2013-02-27 09:31:49 (UTC+8)
出版者:	Camden: Elsevier Ltd
摘要:	An extracellular surfactant-stable chitosanase was purified from the culture supernatant of Bacillus subtilis TKU007 with shrimp shell wastes as the sole carbon/nitrogen source. The TKU007 chitosanase was suppressed by the nattokinase, which appeared at the 1st day. The molecular weight of TKU007 chitosanase was approximately 25 and 33 kDa determined by SDS-PAGE and gel filtration, respectively. The optimum pH, optimum temperature, pH stability and thermal stability of TKU007 chitosanase were pH 7, 37 °C, pH 4–9 and 37 °C, respectively. Closest similarity of TKU007 chitosanase was found on the 226th–229th amino acids of B. subtilis ywtD gamma-dl-glutamyl hydrolase. The amino acid sequence of TKU007 nattokinase was similar to that of B. subtilis nattokinase.
關聯:	Process Biochemistry 43(2), pp.132-138
DOI:	10.1016/j.procbio.2007.11.002
顯示於類別:	[化學學系暨研究所] 期刊論文

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