Purification and characterization of a chitosanase from a nattokinase producing strain Bacillus subtilis TKU007

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Title:	Purification and characterization of a chitosanase from a nattokinase producing strain Bacillus subtilis TKU007
Authors:	Wang, San-Lang;Yeh, Pe-Yi
Contributors:	淡江大學化學學系
Keywords:	Bacillus subtilis;Chitosanase;Nattokinase;Surfactant stable;Shrimp shell wastes
Date:	2008-02
Issue Date:	2013-02-27 09:31:49 (UTC+8)
Publisher:	Camden: Elsevier Ltd
Abstract:	An extracellular surfactant-stable chitosanase was purified from the culture supernatant of Bacillus subtilis TKU007 with shrimp shell wastes as the sole carbon/nitrogen source. The TKU007 chitosanase was suppressed by the nattokinase, which appeared at the 1st day. The molecular weight of TKU007 chitosanase was approximately 25 and 33 kDa determined by SDS-PAGE and gel filtration, respectively. The optimum pH, optimum temperature, pH stability and thermal stability of TKU007 chitosanase were pH 7, 37 °C, pH 4–9 and 37 °C, respectively. Closest similarity of TKU007 chitosanase was found on the 226th–229th amino acids of B. subtilis ywtD gamma-dl-glutamyl hydrolase. The amino acid sequence of TKU007 nattokinase was similar to that of B. subtilis nattokinase.
Relation:	Process Biochemistry 43(2), pp.132-138
DOI:	10.1016/j.procbio.2007.11.002
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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