English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 57064/90742 (63%)
造访人次 : 12481080      在线人数 : 184
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/18867


    题名: Purification and characterization of protease and chitinase from Bacillus cereus TKU006 and conversion of marine wastes by these enzymes
    作者: Wang, San-Lang;Chao, Chia-Hsing;Liang, Tzu-Wen;Chen, Chung-Chih
    贡献者: 淡江大學化學學系
    关键词: Protease;Chitinase;Antioxidant;Shrimp shells;Bacillus cereus
    日期: 2009-06
    上传时间: 2013-02-27 09:32:00 (UTC+8)
    出版者: Secaucus: Springer New York LLC
    摘要: A chitinase- and protease-producing bacterium was isolated and identified as Bacillus cereus TKU006. The better condition on our tests for protease and chitinase production was found when the culture was shaken at 25°C for 2 days in 25 mL of medium containing 2% shrimp shell powder (w/v), 0.1% K2HPO4, and 0.05% MgSO4·7H2O. The molecular masses of TKU006 protease and chitinase determined by sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis were approximately 39 and 35 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of TKU006 protease and chitinase were 9, 50°C, 3–11, 50°C and 5, 40°C, 3–11, 60°C, respectively. TKU006 protease was inhibited completely by EDTA, indicating that the TKU006 protease was a metalloprotease. The TKU006 protease and chitinase retained 61%, 60%, 73%, and 100% and 60%, 60%, 71%, and 96% of its original activity in the presence of 2% Tween 20, 2% Tween 40, 2% Triton X-100, and 1 mM SDS, respectively. The antioxidant activity of TKU006 culture supernatant was determined through the scavenging ability on DPPH with 70% per milliliter. In conclusion, the novelties of the TKU006 protease and chitinase include its high stability to the surfactants and pH. Besides, with this method, we have shown that marine wastes can be utilized to generate a high-value-added product and have revealed its hidden potential in the production of functional foods.
    關聯: Marine Biotechnology 11(3), pp.334-344
    DOI: 10.1007/s10126-008-9149-y
    显示于类别:[化學學系暨研究所] 期刊論文

    文件中的档案:

    档案 大小格式浏览次数
    index.html0KbHTML265检视/开启

    在機構典藏中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回馈