English  |  正體中文  |  简体中文  |  Items with full text/Total items : 57615/91160 (63%)
Visitors : 13534705      Online Users : 341
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/18865

    Title: Conversion of shrimp shell by using Serratia sp. TKU017 fermentation for the production of enzymes and antioxidants
    Authors: Wang, San-Lang;Li, J.Y.;Liang, Tzu-Wen;Hsieh, Jia-Lin;Tseng, Wan-Nine
    Contributors: 淡江大學生命科學研究所
    Keywords: chitinase;protease;antioxidant activity;Serratia sp.;shrimp shell wastes
    Date: 2010-01
    Issue Date: 2013-02-27 09:39:59 (UTC+8)
    Publisher: Seoul: HHan'gug Mi'saengmul Saengmyeong Gong Haghoe
    Abstract: A chitinase (CHT), and a protease (PRO) were purified from the culture supernatant of Serratia sp. TKU017 with shrimp shell as the sole carbon/nitrogen source. The molecular masses of CHT and PRO determined by SDS-PAGE were approximately 65 kDa and 53 kDa, respectively. CHT was inhibited by Mn2+, Cu2+ and PRO was inhibited by most tested divalent metals, EDTA. The optimum pH, optimum temperature, pH stability, and thermal stability of CHT and PRO were (pH 5, 50°C, pH 5–7, <50°C) and (pH 9, 40°C, pH 5–11, <40°C), respectively. PRO retained 95% of its protease activity in the presence of 0.5 mM SDS. The result demonstrates that PRO is SDS-resistant protease and probably has a rigid structure. The 4th day supernatant showed the strongest antioxidant activity (70%, DPPH scavenging ability) and the highest total phenolic content (196±6.2 μg of gallic acid equival/mL). Significant associations between the antioxidant potency and the total phenolic content, as well as between the antioxidant potency and free amino groups, were found for the supernatant. With this method, we have shown that shrimp shell wastes can be utilized and it’s effective in the production of enzymes and antioxidants, facilitating its potential use in industrial applications and functional foods.
    Relation: Journal of Microbiology and Biotechnology 20(1), pp.117-126
    DOI: 10.4014/jmb.0905.05045
    Appears in Collections:[Graduate Institue of Life Sciences] Journal Article

    Files in This Item:

    File Description SizeFormat
    1017-7825_20(1)p117-126.pdf1429KbAdobe PDF311View/Open

    All items in 機構典藏 are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - Feedback