淡江大學機構典藏:Item 987654321/18857
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 62797/95867 (66%)
造访人次 : 3734041      在线人数 : 415
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/18857


    题名: Reversible immobilization of lysozyme via coupling to reversibly soluble polymer
    作者: Sz-Hau Chen;Yue-Horng Yen;Chuan-Lu Wang;San-Lang Wanga
    贡献者: 淡江大學生命科學研究所
    关键词: Lysozyme;Chitinase;Reversibly soluble polymers;Immobilized enzyme
    日期: 2003-10-08
    上传时间: 2009-09-22 15:28:56 (UTC+8)
    出版者: Elsevier
    摘要: Hen egg-white lysozyme (HEWL) can be covalently immobilized on a polymeric support (hydroxypropyl methylcellulose acetate succinate, AS-L), which is soluble above pH 5.5 but insoluble below pH 4.5. Commercial preparation of HEWL was used for the immobilization study. The immobilized enzyme (HEWL-AS) has much higher specific activity than those of free HEWL. The effects of pH and temperature on the activity and thermal stability of HEWL-AS were studied and compared with those of free HEWL. HEWL-AS retained nearly half of its initial chitinase activity even after heating at 70 °C for 50 min. The half-life at 4 °C was 4 days for free HEWL and 25 days for HEWL-AS. Following repeated pH cycles between 7 and 4.5, HEWL-AS retained nearly 50% of its original activity after 10 batches of chitinolytic reactions.
    關聯: Enzyme and Microbial Technology 33(5), p.643-649
    DOI: 10.1016/S0141-0229(03)00186-8
    显示于类别:[化學學系暨研究所] 期刊論文

    文件中的档案:

    档案 大小格式浏览次数
    index.html0KbHTML51检视/开启

    在機構典藏中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回馈