Reversible immobilization of lysozyme via coupling to reversibly soluble polymer

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Title:	Reversible immobilization of lysozyme via coupling to reversibly soluble polymer
Authors:	Sz-Hau Chen;Yue-Horng Yen;Chuan-Lu Wang;San-Lang Wanga
Contributors:	淡江大學生命科學研究所
Keywords:	Lysozyme;Chitinase;Reversibly soluble polymers;Immobilized enzyme
Date:	2003-10-08
Issue Date:	2009-09-22 15:28:56 (UTC+8)
Publisher:	Elsevier
Abstract:	Hen egg-white lysozyme (HEWL) can be covalently immobilized on a polymeric support (hydroxypropyl methylcellulose acetate succinate, AS-L), which is soluble above pH 5.5 but insoluble below pH 4.5. Commercial preparation of HEWL was used for the immobilization study. The immobilized enzyme (HEWL-AS) has much higher specific activity than those of free HEWL. The effects of pH and temperature on the activity and thermal stability of HEWL-AS were studied and compared with those of free HEWL. HEWL-AS retained nearly half of its initial chitinase activity even after heating at 70 °C for 50 min. The half-life at 4 °C was 4 days for free HEWL and 25 days for HEWL-AS. Following repeated pH cycles between 7 and 4.5, HEWL-AS retained nearly 50% of its original activity after 10 batches of chitinolytic reactions.
Relation:	Enzyme and Microbial Technology 33(5), p.643-649
DOI:	10.1016/S0141-0229(03)00186-8
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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