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    Please use this identifier to cite or link to this item: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/18854


    Title: Utilization of squid pen for the efficient production of chitosanase and antioxidants through prolonged autoclave treatment
    Authors: Wang, San-lang;Wu, Pei-Chen;Liang, Tzu-wen
    Contributors: 淡江大學生命科學研究所
    Keywords: Chitosanase;Antioxidant;Squid pen wastes;Bacillus sp.;Autoclave treatment
    Date: 2009-05-26
    Issue Date: 2009-09-22 15:28:45 (UTC+8)
    Publisher: Kidlington: Pergamon
    Abstract: We have developed a culture system for efficient production of chitosanase by Bacillus sp. TKU004. TKU004 was cultivated by using squid pen powder as the sole carbon/nitrogen source. The effects of autoclave treatments of the medium on the production of chitosanase were investigated. Autoclave treatment of squid pen powder for 45 min remarkably promoted enzyme productivity. When the culture medium containing an initial squid pen powder concentration of 3% was autoclaved for 45 min, the chitosanase activity was optimal and reached 0.14–0.16 U/mL. In addition, extracellular surfactant-stable chitosanase was purified from the TKU004 culture supernatant. The antioxidant activity of TKU004 culture supernatant was determined through the scavenging ability of DPPH, with 70% per mL. With this method, we have shown that marine wastes can be utilized efficiently through prolonged autoclave treatments to generate a high value-added product, and have revealed its hidden potential in the production of functional foods.
    Relation: Carbohydrate Research, 344(8), pp.979-984
    DOI: 10.1016/j.carres.2009.03.011
    Appears in Collections:[化學學系暨研究所] 期刊論文

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