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    Title: Degradation of chitin and production of bioactive materials by bioconversion of squid pens
    Authors: Wang, San-Lang;Yang, Chiann-Wen;Liang, Tzu-Wen;Peng, Juo-Hua;Wang, Chuan-Lu
    Contributors: 淡江大學化學學系
    Keywords: Serratia marcescens;Protease;Chitosanase;Squid pen wastes;Deproteinization
    Date: 2009-09
    Issue Date: 2013-02-27 09:31:37 (UTC+8)
    Publisher: Kidlington: Pergamon
    Abstract: Serratia marcescens TKU011, a protease- and chitosanase-producing bacterium, the optimized condition for protease and chitosanase production was found after the media were heated at 121 °C for 120 min and the culture was shaken at 25 °C for 5 days in 100 mL of medium containing 1% squid pen powder (SPP) (w/v), 0.1% K2HPO4, and 0.05% MgSO4. An extracellular metalloprotease with novel properties of solvent stable, and alkaline was purified from the culture supernatant of S. marcescens TKU011 with squid pen wastes as the sole carbon/nitrogen source. The enzyme was a monomeric protease with a molecular mass of 48–50 kDa by SDS–PAGE and gel filtration chromatography. The optimum pH, optimum temperature, pH stability, and thermal stability of TKU011 protease were 8, 50 °C, pH 5–11, and <40 °C, respectively. Besides protease and chitosanase, with this method, deproteinization of squid pen for β-chitin, the production of peptide and reducing sugar may be useful for biological applications.
    Relation: Carbohydrate Polymers 78(2), pp.205-212
    DOI: 10.1016/j.carbpol.2009.03.020
    Appears in Collections:[化學學系暨研究所] 期刊論文

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