Dual-site recognition of sialic acid using an amphiphilic bis‑boronic acid probe in a liquid crystal sensing platform

doi:10.1016/j.microc.2026.117067

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/129198

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Title:	Dual-site recognition of sialic acid using an amphiphilic bis‑boronic acid probe in a liquid crystal sensing platform
Authors:	Nandi, Rajib;Yeh, Yi-Yun;Pan, Po-Shen;Chen, Chih-Hsin
Date:	2026-01-20
Issue Date:	2026-04-16 12:05:38 (UTC+8)
Publisher:	Elsevier
Abstract:	Sialic acid, a terminal monosaccharide present on glycoproteins and glycolipids, plays critical roles in cell adhesion, immune recognition, and metastasis, and its abnormal levels are closely associated with disease progression. Herein, we report a liquid crystal (LC)-based optical sensing platform for label-free detection of sialic acid using a newly designed amphiphilic bis‑boronic acid probe (DIBA) that enables dual-site recognition and enhanced binding affinity. When doped into the nematic liquid crystal 4-cyano-4′-pentylbiphenyl (5CB), DIBA induces homeotropic alignment at the LC/aqueous interface, resulting in a dark optical state. Upon exposure to sialic acid, cooperative binding interactions—primarily boronate ester formation with the α-hydroxycarboxyl group, assisted by hydrogen bonding with the glycerol side chain—disrupt the LC alignment and trigger a distinct dark-to-bright optical transition. The binding mechanism was systematically validated by zeta potential measurements, Fourier transform infrared (FTIR) spectroscopy, binding constant determination, and density functional theory (DFT) calculations. The sensor exhibits excellent selectivity against common monosaccharides, a lowest detectable concentration of 25 μM, and a linear response range of 25–500 μM in buffer. Reliable detection down to 50 μM was further demonstrated in spiked human serum albumin samples, indicating good tolerance toward complex biological matrices. This work represents the first LC-based sensing platform for sialic acid detection employing a bis‑boronic acid amphiphilic probe, providing a simple, non-enzymatic, and visually readable approach for bioanalytical sensing applications.
Relation:	Microchemical Journal 221 ,p. 117067
DOI:	10.1016/j.microc.2026.117067
Appears in Collections:	[化學學系暨研究所] 期刊論文

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