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https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/126419
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| 题名: | Metabolic engineering of the borneol and camphor degradation pathways in Pseudomonas to produce optically pure bicyclic monoterpenes |
| 作者: | Lee, Pei-yun;Hong, Wei-xiang;Shih, Tzenge-lien |
| 日期: | 2024-10-16 |
| 上传时间: | 2024-10-21 12:05:33 (UTC+8) |
| 摘要: | Borneol, a medicinally important bicyclic monoterpene, facilitates drug transport across mucous membranes and the blood-brain barrier. Derivatives of borneol and camphor also have numerous biomedical applications. Borneol is currently industrially synthesized via the conversion of turpentine and α-pinene. However, the major product is racemic isoborneol rather than racemic borneol. Both borneol and isoborneol are degraded by the soil bacterium Pseudomonas via a well-established degradation pathway. Two indigenous Pseudomonas strains were used to convert racemic isoborneol to other optically pure bicyclic monoterpenes here. Our results showed that deletion of the camE2,5 gene alone from the strain TCU-HL1 genome led to the complete loss of borneol and camphor degradation ability. Knockout of both camE2,5 and bdh1 (TCU-HL1Δbdh1ΔcamE2,5) restored the degradation capability as the role of Bdh1 was replaced by that of Bdh2. This mutant converted racemic isoborneol into an optically pure bicyclic monoterpene, 2,5-diketocamphane, with a 45 % recovery yield. RT-qPCR results suggested that camE2,5 expression plays a pivotal role in regulating the borneol/camphor degradation cluster. While (+)-borneol, (–)-borneol and (+)-camphor can be obtained from plants for mass production purposes, (–)-camphor cannot be obtained in the same manner. P. monteilii TCU-CK1 converted racemic isoborneol into (–)-camphor and 3,6-diketocamphane, with 15 % and 10 % recovery yields, respectively. In conclusion, we report the role of camE2,5 in regulating the borneol/camphor degradation operon and biotransformation methods to produce several optically pure bicyclic monoterpenes. |
| 關聯: | Enzyme and Microbial Technology 181, 110527 |
| DOI: | 10.1016/j.enzmictec.2024.110527 |
| 显示于类别: | [應用科學博士班] 期刊論文
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