Injectable ChitHCl-MgSO4-DDA hydrogel as a bone void filler to improve cell migration and osteogenesis for bone regeneration

doi:10.1016/j.ijbiomac.2024.132409

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Title:	Injectable ChitHCl-MgSO4-DDA hydrogel as a bone void filler to improve cell migration and osteogenesis for bone regeneration
Authors:	Wang, Yu-tzu
Date:	2024-05-18
Issue Date:	2024-08-07 12:06:19 (UTC+8)
Abstract:	Suture pull-through is a clinical problem in meniscus repair surgery due to the sharp leading edge of sutures. Several tissue adhesives have been developed as an alternative to traditional suturing; however, there is still no suitable tissue adhesive specific for meniscus repair treatment due to unsatisfactory biosafety, biodegradable, sterilizable, and tissue-bonding characteristics. In this study, we used a tissue adhesive composed of chitosan hydrochloride reacted with oxidative periodate-oxidized dextran (ChitHCl-DDA) combined with a chitosan-based hydrogel and oxidative dextran to attach to the meniscus. We conducted viscoelastic tests, viscosity tests, lap shear stress tests, Fourier transform infrared (FTIR) spectroscopy, swelling ratio tests, and degradation behavior tests to characterize these materials. An MTT assay, alcian blue staining, migration assay, cell behavior observations, and protein expression tests were used to understand cell viability and responses. Moreover, ex vivo and in vivo tests were used to analyze tissue regeneration and biocompatibility of the ChitHCl-DDA tissue adhesive. Our results revealed that the ChitHCl-DDA tissue adhesive provided excellent tissue adhesive strength, cell viability, and cell responses. This tissue adhesive has great potential for torn meniscus tissue repair and regeneration.
Relation:	International Journal of Biological Macromolecules 270(2),　132409
DOI:	10.1016/j.ijbiomac.2024.132409
Appears in Collections:	[Graduate Institute & Department of Mechanical and Electro-Mechanical Engineering] Journal Article

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