Since the yields of trading stocks are affected by numerous elements, this study aims to explore whether the profitability of trading stock with the use of technical trading rules, such as Stochastic Oscillator Indicators (SOI) and Relative Strength Index (RSI), would matter in the fourth quarter of the year. By employing the data of Dow Jones 30 (DJI 30) and NASDAQ 100 (NDX100) from 2011 to 2020 and from 1991 to 2020, this study reports that investors applying SOI and RSI would have higher cumulative abnormal returns (CARs) in Quarter 4 as compared with those in Quarters 1–3, respectively. The revealed findings indicate that the quarterly effect should be taken into account by investors when trading DJI 30 and NDX100, the representative indices for US stock markets. More importantly, this study may contribute to the existing literature due to the rare discussion of this interesting issue in the past research.