In situ encapsulation of camptothecin by self-assembly of poly(acrylic acid)-b-poly(N-isopropylacrylamide) and chitosan for controlled drug delivery

doi:10.3390/polym15112463

淡江大學機構典藏 > 工學院 > 化學工程與材料工程學系暨研究所 > 期刊論文 > Item 987654321/124778

請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/124778

題名:	In situ encapsulation of camptothecin by self-assembly of poly(acrylic acid)-b-poly(N-isopropylacrylamide) and chitosan for controlled drug delivery
作者:	Huang, Yi-Cheng;Zeng, Yang-Jie;Lin, Yu-Wei;Tai, Hung-Chih;Don, Trong-Ming
關鍵詞:	stimuli-responsive polymers;poly(acrylic acid);poly(N-isopropylacrylamide);chitosan;camptothecin;drug delivery
日期:	2023-05-26
上傳時間:	2023-12-04 12:05:15 (UTC+8)
出版者:	MDPI AG
摘要:	Camptothecin (CPT) has been shown to exhibit anticancer activity against several cancers. Nevertheless, CPT is very hydrophobic with poor stability, and thus its medical application is limited. Therefore, various drug carriers have been exploited for effectively delivering CPT to the targeted cancer site. In this study, a dual pH/thermo-responsive block copolymer of poly(acrylic acid-b-N-isopropylacrylamide) (PAA-b-PNP) was synthesized and applied to encapsulate CPT. At temperatures above its cloud point, the block copolymer self-assembled to form nanoparticles (NPs) and in situ encapsulate CPT, owing to their hydrophobic interaction as evidenced by fluorescence spectrometry. Chitosan (CS) was further applied on the surface through the formation of a polyelectrolyte complex with PAA for improving biocompatibility. The average particle size and zeta potential of the developed PAA-b-PNP/CPT/CS NPs in a buffer solution were 168 nm and −30.6 mV, respectively. These NPs were still stable at least for 1 month. The PAA-b-PNP/CS NPs exhibited good biocompatibility toward NIH 3T3 cells. Moreover, they could protect the CPT at pH 2.0 with a very slow-release rate. At pH 6.0, these NPs could be internalized by Caco-2 cells, followed by intracellular release of the CPT. They became highly swollen at pH 7.4, and the released CPT was able to diffuse into the cells at higher intensity. Among several cancer cell lines, the highest cytotoxicity was observed for H460 cells. As a result, these environmentally-responsive NPs have the potential to be applied in oral administration.
關聯:	Polymers 15(11), 2463
DOI:	10.3390/polym15112463
顯示於類別:	[化學工程與材料工程學系暨研究所] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	104	檢視/開啟

在機構典藏中所有的資料項目都受到原著作權保護.

TAIR相關文章

資料載入中.....