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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/123413

    题名: Protective role of Bu-Zhong-Yi-Qi decoction on aristolochic acid-intoxicated zebrafish
    作者: Tsai, Jen-Ning;Ding, Yu-Ju;Lo, Kang-Chieh;Lu, Ping-Hsun;Chang, Chiung-Fang;Wen, Chi-Chung;Wang, Bo-Cheng;Yang, Ling-Ling;Chen, Yau-Hung
    关键词: Bu-Zhong-Yi-Qi Decoction;Zebrafish;Kidney;Aristolochic acid;Nephroprotection
    日期: 2021-03-31
    上传时间: 2023-04-28 17:58:36 (UTC+8)
    出版者: Pharmacotherapy Group
    摘要: Purpose: To investigate the potential nephroprotective effects of a traditional Chinese medical prescription, Bu-Zhong-Yi-Qi Decoction (BZYQD) in an aristolochic acid (AA)-intoxicated zebrafish model.
    Methods: A green fluorescent zebrafish line Tg (wt1b:EGFP) was used, and different exposure protocols were applied. Once a suitable protective concentration of BZYQD was found, antibody staining and real-time PCR methods were applied and the pro-inflammatory gene expressions were determined.
    Results: The results showed that low dose (10 ppm) of BZYQD attenuates the AA-induced malformed kidney phenotype. This finding was further substantiated by an examination of the integrity of pronephric tubes in the treated embryos. Pre-treatment with BZYQD suppressed the elevated expressions of pro- inflammatory genes, including tumor necrosis factor-α and myeloperoxidase, induced by AA exposure (1.6 ~ 2.3-fold). This indicates that the nephroprotective effect of BZYQD may be mediated by suppression of pro-inflammatory gene expression. On the other hand, a high dose (500 ppm) of BZYQD caused nephrotoxic effect.
    Conclusion: An efficient model to identify AA-protective compounds using zebrafish embryos has been successfully established. Using this strategy, it has been found that BZYQD is nephroprotective in zebrafish embryos, and might have the potential to be applied in humans.
    關聯: Tropical Journal of Pharmaceutical Research 20(3), p.605-611
    DOI: 10.4314/tjpr.v20i3.24
    显示于类别:[化學學系暨研究所] 期刊論文


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