|摘要: ||目的：本研究目的為探討植物乳桿菌PS128服用對半程馬拉松跑者運動表現肌肉損傷抗氧化壓力的影響。方法：本實驗採用雙盲、隨機、安慰劑對照、對抗平衡及交叉試驗設計。HM在服用4週營養補充劑開始前及結束後進行。八名休閒跑者每日早晚飯前服用兩粒PS128膠囊（3 x 1010 CFU/膠囊），持續4週為PS128介入 (LT)，或者服用兩粒安慰劑膠囊，持續4週作為安慰劑組 (PT)。兩組均在給藥前進行運動能力檢測（下肢肌力、下肢爆發力、無氧爆發力和有氧能力）和血液檢測（肌肉疲勞、肌肉損傷、氧化應激和腎臟損傷等指標）。LT與PT皆於服用營養補充劑之前 (baseline)、HM介入前48小時 (pre)、HM介入後之立即 (0 h post)、3小時 (3 h post)、24小時 (24 h post)、48小時 (48 h post)、72小時 (72 h post) 及96小時 (96 h post) 進行一次運動能力檢測與血液檢測。結果：PT和LT之間的HM完成總時間沒有顯著差異，但在HM 的第4階段 (15,751至21,000公尺) 發現LT顯著低於PT (2,778 ± 551秒vs. 3,394 ± 727秒, p = 0.02)。使用等速肌力檢測儀發現LT的下肢肌力在72 h post顯著高於PT。以下蹲跳檢測發現PT的下肢爆發力在HM介入後的24小時之內顯著下降。而兩組之間的無氧爆發力與有氧能力在HM介入後皆無顯著差異。HM介入後發現LT具有較低的肌肉損傷指標，如肌紅素 (3 h post-PT vs. -LT: 190.6 ± 118 ng/mL vs. 91.7 ± 68.6 ng/mL, p < 0.0001) 及肌酸磷化酶 (24 h post-PT vs. -LT: 875.8 ± 572.3 IU/L vs. 401 ± 295.7 IU/L, p < 0.0001)。LT的血中尿素氮於24小時後即恢復至正常水平 (24 h pre- vs. post-LT, p > 0.05) 及在96小時後有較多的抗氧化指標超氧化物歧化酶 (96 h post-PT vs. -LT: 0.267 ± 0.088 U/mL vs. 0.462 ± 0.122 U/mL, p < 0.0001)。結論：服用PS128營養補充劑4週後可提升HM的運動表現及有效改善HM介入後所帶來的肌肉損傷及抗氧化能力。
Purpose: The study was designed to investigate effects of lactobacillus plantarum PS128 (PS128) intaking on performance, muscle damage and antioxidant stress of half-marathon runners. Methods: A double-blind, randomized, placebo-controlled, counterbalanced, crossover trial was used for the experiment. HM was con-ducted at the beginning and end of the 4-week nutritional supplement administration. Eight recreational runners took two capsules (3 × 1010 CFU/capsule) of PS128 each morning and evening before meals for 4 weeks as the PS128 treatment (LT), or they took two capsules of placebo for 4 weeks as the placebo treatment (PT). In both treatments, an exercise capacity test (lower extremity muscle strength, anaerobic power, lower extremity explosive force, and aerobic capacity) and blood test (muscle fatigue, muscle damage, antioxidative stress, and renal injury) were performed before the administration of the nutritional supplement (baseline), 48 h before HM (pre), and 0 h (0 h post), 3 h (3 h post), 24 h (24 h post), 48 h (48 h post), 72 h (72 h post), and 96 h (96 h post) after HM. Results: There was no significant difference in the total duration of HM between PT and LT, but LT was found to be significantly lower than PT at stage 4 (15,751–21,000 m) of HM (2,778 ± 551 s vs. 3,394 ± 727 s, p = 0.02). The lower extremity muscle strength measured using an isokinetic dynamometer in LT was significantly higher than that in PT at 72 h after HM. The lower extremity explosive force from the countermovement jump (CMJ) in PT was significantly decreased compared to 24 h prior. There was no significant difference between anaerobic power and aerobic capacity between the two treatments after HM. After HM, LT had lower muscle damage indices, such as myoglobin (3 h post-PT vs. -LT: 190.6 ± 118 ng/mL vs. 91.7 ± 68.6 ng/mL, p < 0.0001) and creatine phosphokinase (24 h post-PT vs. -LT: 875.8 ± 572.3 IU/L vs. 401 ± 295.7 IU/L, p < 0.0001). Blood urea nitrogen recovered in 24 h (24 h pre- vs. post-LT, p > 0.05) and higher superoxide dismutase was found in LT (96 h post-PT vs. -LT: 0.267 ± 0.088 U/mL vs. 0.462 ± 0.122 U/mL, p < 0.0001). Conclusions: Taking PS128 nutritional supplements for 4 weeks can improve the exercise performance of HM and effectively improve the muscle damage and antioxidative stress after HM intervention.