Purpose: The different soccer position may influence the body composition. The present study was to investigate the effect of different soccer position on body composition in trained soccer athletes. Method: The open No.1 level healthy male soccer players were recruited to this study. Their training age were 9.0±3 years and regular training time were 15.0 ± 3.0 hours/week. The players were differentiated by different position into: 11 strikers, 12 midfielders and 12 defenders. After overnight fasting, the player arrived the lab. After measured their height, the body composition was measured by BIA method. The analysis of variance was used to analysis the different on body composition. The post hoc test was bonferroni test. Result: the defenders (176.8±6.8cm) were significantly higher than midfielders (169.0±7.0cm) (P=0.029), but there was no different with strikers (P=0.315). The body weight, lean body mass, muscle mass, dominant foot mass and non-dominant foot mass in defenders were significantly higher than midfielders and strikers(P<0.05). There were no different in % body fat, visceral adiposity, dominant hand mass and non-dominant hand mass (P>0.05). Conclusion: Defenders have heavier muscles and lower body weight than strikers and midfielders. The different position training may not change the body composition in soccer players.