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https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/121513
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| 題名: | Utilization of Cassava Wastewater for Low-Cost Production of Prodigiosin via Serratia marcescens TNU01 Fermentation and Its Novel Potent α-Glucosidase Inhibitory Effect |
| 作者: | TL, Tran;Techato, K;VB, Nguyen;SL, Wang;AD, Nguyen;TQ, Phan;MD, Doan;Phoungthong, K |
| 關鍵詞: | cassava wastewater;fermentation;bioreactor;S. marcescens;prodigiosin;α-glucosidase inhibitor;anti-diabetes;docking study |
| 日期: | 2021-10-16 |
| 上傳時間: | 2021-10-19 12:10:40 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | The purpose of this study was to reuse cassava wastewater (CW) for scaled-up production, via the fermentation of prodigiosin (PG), and to conduct an evaluation of its bioactivities. PG was produced at the yield of high 6150 mg/L in a 14 L-bioreactor system, when the designed novel medium (7 L), containing CW and supplemented with 0.25% casein, 0.05% MgSO4, and 0.1% K2HPO4, was fermented with Serratia marcescens TNU01 at 28 °C in 8 h. The PG produced and purified in this study was assayed for some medical effects and showed moderate antioxidant, high anti-NO (anti-nitric oxide), and potential α-glucosidase inhibitory activities. Notably, PG was first reported as a novel effective α-glucosidase inhibitor with a low IC50 value of 0.0183 µg/mL. The commercial anti-diabetic drug acarbose was tested for comparison and had a lesser effect with a high IC50 value of 328.4 µg/mL, respectively. In a docking study, the cation form of PG (cation-PG) was found to bind to the enzyme α-glucosidase by interacting with two prominent amino acids, ASP568 and PHE601, at the binding site on the target enzyme, creating six linkages and showing a better binding energy score (−14.6 kcal/mol) than acarbose (−10.5 kcal/mol). The results of this work suggest that cassava wastewater can serve as a low-cost raw material for the effective production of PG, a potential antidiabetic drug candidate. |
| 關聯: | Molecules 26(20), 6270 |
| 顯示於類別: | [化學學系暨研究所] 期刊論文
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