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| 題名: | Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source |
| 作者: | Doan, Chien Thang;Tran, Thi Ngoc;Nguyen, Thi Thanh;Tran, Thi Phuong Hanh;Nguyen, Van Bon;Tran, Trung Dung;Nguyen, Anh Dzung;Wang, San-Lang |
| 關鍵詞: | agro-byproduct;Bacillus licheniformis;bioconversion;pomelo albedo;sucrolytic |
| 日期: | 2021-06-13 |
| 上傳時間: | 2021-08-02 12:10:19 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | Recently, there has been increasing use of agro-byproducts in microbial fermentation to
produce a variety of value-added products. In this study, among various kinds of agro-byproducts,
pomelo albedo powder (PAP) was found to be the most effective carbon source for the production of
sucrose hydrolyzing enzyme by Bacillus licheniformis TKU004. The optimal medium for sucrolytic
enzyme production contained 2% PAP, 0.75% NH4NO3
, 0.05% MgSO4
, and 0.05% NaH2PO4 and the
optimal culture conditions were pH 6.7, 35 ◦C, 150 rpm, and 24 h. Accordingly, the highest sucrolytic
activity was 1.87 U/mL, 4.79-fold higher than that from standard conditions using sucrose as the
carbon source. The purified sucrolytic enzyme (sleTKU004) is a 53 kDa monomeric protein and
belongs to the glycoside hydrolase family 68. The optimum temperature and pH of sleTKU004 were
50 ◦C, and pH = 6, respectively. SleTKU004 could hydrolyze sucrose, raffinose, and stachyose by
attacking the glycoside linkage between glucose and fructose molecules of the sucrose unit. The Km
and Vmax of sleTKU004 were 1.16 M and 5.99 µmol/min, respectively. Finally, sleTKU004 showed
strong sucrose tolerance and presented the highest hydrolytic activity at the sucrose concentration of
1.2 M–1.5 M. |
| 關聯: | Polymers 13(12), 1959 |
| DOI: | 10.3390/polym13121959 |
| 顯示於類別: | [化學學系暨研究所] 期刊論文
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