Bio-processing of marine chitinous wastes for the production of bioactive prodigiosin

doi:10.3390/molecules26113138

機構典藏 > College of Sciences > Graduate Institute & Department of Chemistry > Journal Article > Item 987654321/120839

Please use this identifier to cite or link to this item: https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/120839

Title:	Bio-processing of marine chitinous wastes for the production of bioactive prodigiosin
Authors:	TH, Nguyen;SL, Wang;DM, Nguyen;AD, Nguyen;TH, Nguyen;MD, Doan;VA, Ngo;CT, Doan;YH, Kuo;VB, Nguyen
Keywords:	prodigiosin;Serratia marcescens;shrimp shells;bioreactor;fermentation;antioxidants;anti‐NO activity
Date:	2021-05-24
Issue Date:	2021-06-10 12:12:31 (UTC+8)
Publisher:	MDPI
Abstract:	Recently, microbial prodigiosin (PG) has received much attention due to its numerous beneficial applications. The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost‐effective preparation of PG. Of the MCWs, demineralized shrimp shell powders (de‐SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using Serratia marcescens strains. Further, PG scale‐up production was in‐ vestigated in a 15 L bioreactor system, and the highest yield (6200 mg/L) was achieved during fer‐ mentation using 5 L of a novel‐designed culture broth that included 1.60% C/N sources (a de‐SSP/ca‐ sein ratio of 7/3), 0.02% K2SO4, and 0.05% K2HPO4, with an initial pH of 6–7. Fermentation was con‐ ducted in the dark at 27.5 °C for 8.0 h. This study was the first to report on the utilization of shrimp wastes for cost‐effective, large‐scale (5 L/pilot) PG production with high productivity (6200 mg/L) in a short cultivation time. The combination of 0.02% K2SO4 and 0.05% K2HPO4 was also found to be a novel salt composition that significantly enhanced PG yield. The red compound was purified and confirmed as PG after analyzing its HPLC profile, mass, and UV/vis spectra. The purified PG was then tested for its bioactivities and showed effective anticancer activities, moderated antioxi‐ dant activities, and novel anti‐NO effects.
Relation:	Molecules 26 (11), 3138
DOI:	10.3390/molecules26113138
Appears in Collections:	[Graduate Institute & Department of Chemistry] Journal Article

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