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    题名: A sensitive bithiophene-based biosensor for interferon-gamma characterization and analysis
    作者: Tsai, P.I.;Lee, S.S.;Chou, S.T.;Jan, C.M.;Chang, Y.T.;Lee, A.S.Y.;Lee, C.K.
    关键词: Surface plasmon resonance;Ellipsometry;Interferon-gamma;Phase-interrogation;Bithiophene
    日期: 2014-11-28
    上传时间: 2021-05-08 12:10:51 (UTC+8)
    出版者: Elsevier BV
    摘要: Real-time, label-free monitoring offers a wide range of applications to the biotech field. We developed an integrated bio-sensing platform which adapts a circular polarization interferometry configuration and a phase modulated ellipsometer to improve the performance of biomolecular measurements. An bithiophene-based conductive linker-5′-(mercapto)-[2, 2′-bithiophene]-5-carboxylic acid was developed for detecting antibody–antigen interactions. In this study, the biomarker interferon-gamma (IFN-γ), one of the most important indicators of tuberculosis, was chosen to test and verify the sensitivity of our measurement system. Our experimental results showed that an increase in the concentration of the IFN-γ (64 pM to 1 μM) was usually accompanied by a phase increase. This result is good evidence that our biosensing system can be useful for analyzing biomolecular interactions.
    關聯: Thin Solid Films 571(3), p.473-477
    DOI: 10.1016/j.tsf.2013.12.037
    显示于类别:[化學學系暨研究所] 期刊論文

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