Highly sensitive distance-based liquid crystalline visualization for paper-based analytical devices

doi:10.1016/j.aca.2021.338328

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/120684

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题名:	Highly sensitive distance-based liquid crystalline visualization for paper-based analytical devices
作者:	Khachornsakkul, K.;Chang, J.-J.;Lin, P.-H.;Lin, Y.-H.;Dungchai, W.;Chen, C.-H.
关键词:	Bilirubin;Liquid crystal;Ultrasound;Distance-based sensors;Paper-based sensors
日期:	2021-02-18
上传时间:	2021-04-30 12:10:43 (UTC+8)
出版者:	Elsevier
摘要:	We successfully report on the first demonstration of a highly sensitive distance-based liquid crystalline visualization for paper-based analytical devices. The construction of this paper sensor was achieved by immobilizing 4-cyano-4′-pentylbiphenyl (5CB) as liquid crystalline molecules (LCs) onto a paper strip substrate. The sensing mechanism is based on the ultrasound-assisted decomposition of 5CB by the hydroxyl radical (•OH) which is generated from the oxidase enzymatic reaction of the analyte, this then results in the change of texture and color of paper. The utility of our devices was then demonstrated with the determination of bilirubin (BR) in biological samples using a bilirubin oxidase enzymatic reaction. The quantification of BR can be achieved by dipping the tip of the paper strips into the analyte solutions and then by measuring the length of color which has been changed on the paper, by the naked eye. Under optimized conditions, this paper sensor offered the linear range of BR detection from 2.0 to 30.0 pmol/L (R2 = 0.9945) with the limit of detection (LOD) of 0.80 pmol/L. In addition, the results of this sensor were highly reproducible, with a relative standard deviation (RSD) of less than 3.50%. The recoveries of spiked BR in human urine and serum samples were in the range of 99.09–107.89%, which demonstrates the high accuracy of this paper sensor. Overall, this work presents a simple method to determine the concentration of H2O2 and BR at pmol levels with an instrument-free length-measuring readout, so it could be suitable for quantitative analysis of other biomarkers based on oxidase enzymatic reaction, which can provide important information about early disease diagnosis and patient prognosis.
關聯:	Analytica Chimica Acta 1154, 338328
DOI:	10.1016/j.aca.2021.338328
显示于类别:	[化學學系暨研究所] 期刊論文

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