The Effect of Disulfide Bonds on Protein Folding, Unfolding, and Misfolding Investigated by FT-Raman Spectroscopy

doi:10.1002/jrs.4935

淡江大學機構典藏 > 理學院 > 化學學系暨研究所 > 期刊論文 > Item 987654321/120056

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题名:	The Effect of Disulfide Bonds on Protein Folding, Unfolding, and Misfolding Investigated by FT-Raman Spectroscopy
作者:	Wang, Chih-Hsien;Huang, Chia-Chi;Lin, Long-Liu;Chen, Wenlung
关键词:	disulfide bonds;FT–Raman;BSA;secondary structure;folding/unfolding
日期:	2016-08-06
上传时间:	2021-03-05 12:14:29 (UTC+8)
摘要:	Disulfide bond is relevant to many protein folding/unfolding functions and conformational diseases. To elucidate the effects of disulfide bonds on protein folding, unfolding, and misfolding, we performed Fourier transform–Raman measurements on serial chemical‐induced denaturations of bovine serum albumin (BSA). By directly monitoring Raman stretching at S–S (~507 cm−1), S–H (~2566 cm−1), amide I (1655 cm−1 for α‐helix; 1667 cm−1 for β‐sheet structure), and amide III (>1300 cm−1 for α‐helix; 1246 cm−1 for β‐sheet structure), the status of disulfide bonds and secondary structure of BSA at different states were elucidated. Both disulfide bonds and secondary structure (mostly in α‐helix) of BSA appeared relatively stable even when the protein was unfolded by urea solution. However, disulfide bonds were completely reduced and protein secondary structure changed from α‐helix to a relatively β‐sheet dominant when the protein was modified by the mixed solution of urea and dithiothreitol (urea/DTT). Adhering to these structural changes, the protein proceeded to different degrees of polymerization. BSA would aggregate into a high molecular mass (over 700 kDa) of protein ensemble when it was exposed to the mixed urea/DTT solution. An irreversible change in S–S/S–H conversion and secondary structure was responsible for protein misfolding. We demonstrate here that Fourier transform–Raman directly probe S–S/S–H conversion and secondary structural change of BSA at different states, and these results clearly indicate that disulfide bonds and secondary structure of BSA serve as concrete frameworks to stabilize protein structure. As the frameworks collapse, the protein undergoes an irreversible structural change and results in protein misfolding.
關聯:	Journal of Raman Spectroscopy 47(8), p.940-947
DOI:	10.1002/jrs.4935
显示于类别:	[化學學系暨研究所] 期刊論文

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