English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 58286/91808 (63%)
造訪人次 : 13813537      線上人數 : 66
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library & TKU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/120051


    題名: Label-Free SERS Characterization of Snake Venoms by Exploring the Cysteine Environs with Bone-Shaped Gold Nanoparticles
    其他題名: English
    作者: Huang, Chia-Chi;Lai, Yen-Shi;Wang, Chih-Hsien;Chau, Lai-Kwan;Chen, Wenlung
    日期: 2020-11-04
    上傳時間: 2021-03-05 12:14:20 (UTC+8)
    摘要: Identification of snake venoms is a vital step in the treatment of fatal snakebites. In this study, we use the gold–thiolate interaction between a cysteine residue and gold nanoparticles to establish a SERS method for the differentiation of the venoms of Trimeresurus stejnegeri and Bungarus multicinctus. We confirm the preference of gold nanoparticles over silver for the SERS study of snake venoms by a binding experiment that also functions to differentiate the two venom samples by colorimetry and UV-vis spectroscopy. We report the SERS spectra of Trimeresurus stejnegeri and Bungarus multicinctus venoms for the first time. The spectra display distinct SERS signatures of the snake venoms on bone-shaped gold nanoparticles made with a house recipe. These signatures correlate to selected segments of the venom proteins due to the anchoring effect of the gold-cysteine bond. The method is quick as it accomplishes in situ isolation of the structure of interest to avoid tedious purification of the samples. The location of the interactive cysteine residue makes a novel characteristic of proteins in general.
    關聯: Journal of Material Chemistry B 8(47), p.10744-10753
    DOI: 10.1039/D0TB02443K
    顯示於類別:[化學學系暨研究所] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML7檢視/開啟

    在機構典藏中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library & TKU Library IR teams. Copyright ©   - 回饋