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https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/119850
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| 題名: | Conversion of wheat bran to xylanases and dye adsorbent by Streptomyces thermocarboxydus |
| 作者: | TN, Tran;CT, Doan;SL, Wang |
| 關鍵詞: | Agro-byproducts;dye adsorption;antioxidant;prebiotic;Streptomyces thermocarboxydus;xylanase;xylo-oligosaccharide |
| 日期: | 2021-01-17 |
| 上傳時間: | 2021-01-18 12:10:39 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | Agro-byproducts can be utilized as effective and low-cost nutrient sources for microbial fermentation to produce a variety of usable products. In this study, wheat bran powder (WBP) was found to be the most effective carbon source for xylanase production by Streptomyces thermocarboxydus TKU045. The optimal media for xylanase production was 2% (w/v) WBP, 1.50% (w/v) KNO3, 0.05% (w/v) MgSO4, and 0.10% (w/v) K2HPO4, and the optimal culture conditions were 50 mL (in a 250 mL-volume Erlenmeyer flask), initial pH 9.0, 37 °C, 125 rpm, and 48 h. Accordingly, the highest xylanase activity was 6.393 ± 0.130 U/mL, 6.9-fold higher than that from un-optimized conditions. S. thermocarboxydus TKU045 secreted at least four xylanases with the molecular weights of >180, 36, 29, and 27 kDa when cultured on the WBP-containing medium. The enzyme cocktail produced by S. thermocarboxydus TKU045 was optimally active over a broad range of temperature and pH (40–70 °C and pH 5–8, respectively) and could hydrolyze birchwood xylan to produce xylobiose as the major product. The obtained xylose oligosaccharide (XOS) were investigated for 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the growth effect of lactic acid bacteria. Finally, the solid waste from the WBP fermentation using S. thermocarboxydus TKU045 revealed the high adsorption of Congo red, Red 7, and Methyl blue. Thus, S. thermocarboxydus TKU045 could be a potential strain to utilize wheat bran to produce xylanases for XOS preparation and dye adsorbent. |
| 關聯: | Polymers 13(2), 287 |
| DOI: | 10.3390/polym13020287 |
| 顯示於類別: | [化學學系暨研究所] 期刊論文
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