淡江大學機構典藏:Item 987654321/118772
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    题名: Microbial reclamation of chitin and protein-containing marine by-products for the production of prodigiosin and the evaluation of its bioactivities
    作者: Nguyen, Van Bon;Nguyen, Dai Nam;Wang, San‐Lang
    关键词: β-chitin;protein;prodigiosin;bioconversion;anticancer;AChE inhibitor;bioreactor
    日期: 2020-06-10
    上传时间: 2020-06-11 12:10:15 (UTC+8)
    出版者: MDPI
    摘要: Chitin and protein-containing marine by-products (CPCMBPs), including crab shells, squid pens, and shrimp shells, were investigated as the sole carbon/nitrogen (C/N) source for prodigiosin (PG) production by Serratia marcescens TNU01 in a 250 mL Erlenmeyer flask and a 10 L bioreactor system. Among the used C/N source of CPCMBPs, squid pens powder (SPP) showed the most optimum PG productivity. Different ratios of chitin/protein combination were also used as the C/N sources for PG production. With a similar chitin/protein ratio (4/6) of squid pens, a significant PG productivity was achieved when the chitin/protein ratios were controlled in the range of 3/7–4/6. Maximum PG yield (3450 mg/L) by S. marcescens TNU01 was achieved in the bioreactor system containing 3 L medium of 1.75% SPP, 0.03% K2HPO4, and 0.05% MgSO4 at 25 °C for 12 h in dark. The results of in vitro bioassays reveal that the purified PG possesses acetylcholinesterase inhibitory activity and antioxidant as well as anticancer activities. This study suggests that squid pens may have the potential to be used for cost effective production of bioactive PG at a large-scale.
    關聯: Polymers 12(6), 1328
    显示于类别:[化學學系暨研究所] 期刊論文

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